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Parasitology and Parasitological Diseases

SCI papers since 2009

Publications of the "Parasitology and Parasitological Diseases Lab" team members



Amairia, S., Rouatbi, M., Rjeibi, M.R., Gomes, J., Rekik, M., Darghouth, M.A., Gharbi, M., 2021. Molecular detection of Toxoplasma gondii and Sarcocystis spp. co-infection in Tunisian Merguez, a traditional processed sausage beef meat. Food Control, 121. 

Baptista, C.J., Sós, E., Madeira de Carvalho, L.M., 2021. Gastrointestinal parasitism in Przewalski horses (Equus ferus przewalskii) – a review. Acta Parasitol, 1-7. 

Cardoso, B., Pessoa, B., Figueiredo, P., Rinaldi, L., Cringoli, G., Díaz, A., Gomes, L., Santos, N., Madeira De Carvalho, L.M., 2021. Comparative survey of gastrointestinal parasites in sympatric Iberian ibex (Capra pyrenaica) and domestic goats using molecular host specific identification. Parasitol Res, 120 (5) 1-6.

Dordio, A.M.,  Beck, R., Nunes, T., Pereira da Fonseca, I., Gomes, J., 2021. Molecular survey of vector-borne diseases in two groups of domestic dogs from Lisbon, Portugal. Parasites Vectors 14:163. 

Figueiredo, A.M., Madeira de Carvalho, L.M., Palacios, M.J., Pla, S., Núñez-Arjona, J.C., Rueda, C., Vallverdú-Coll, N., Silvestre, F., Peña, J., Calero-Bernal, R., Carmena, D., Ángel Habela, M., Fonseca, C., Tinoco Torres, R., Nájera, F., 2021. Parasites shared between the reintroduced Iberian lynx (Lynx pardinus) and sympatric mesocarnivores in Extremadura, Spain. Pathogens, 10, 274-285.

Louro, M., Kuzmina, T.A., Bredtmann, C.M., Dieckmann, I., Madeira de Carvalho, L.M., Samson-Himmelstjerna, G., Krücken, J., 2021. DNA barcoding and phylogenetic analysis of six common cyathostomin species based on mitochondrial (COI) and ITS-2 genes. Scientific Reports, 11:8245-8254. 

Pinto-Bravo, P., Rebordão M. R., Amaral, A., Fernandes, C., Galvão, A., Silva, E., Pessa-Santos, P., Alexandre-Pires, G., Roberto da Costa, R.P., Skarzynski, D.J. and Ferreira-Dias, G., 2021. Microvascularization and Expression of Fibroblast Growth Factor and Vascular Endothelial Growth Factor and Their Receptors in the Mare Oviduct. Animals, 11, 1099

Ramilo, D.W.,  Caetano, I., Brazio, E., Mira, M., Antunes, L., Pereira da Fonseca, I., Cardoso, L., 2021. Presence of one ecto- and two endoparasite species of the black stork (Ciconia nigra) in Portugal. BMC Veterinary Research 17:21. 

Ramilo, D.W., Costa, P., Stekolnikov, A.A., Cláudio, J.M., Lourenço, A.M.,  Pereira da Fonseca, I., Cardoso, L., 2021.  First report of Ericotrombidium ibericense in domestic dogsActa Parasitologica, 66(1): 253-258, 2021, ISSN 1230-2821.

Rousseau, J., Nakamura, M., Rio-Maior, H., Álvares, F., Choquet, R., Madeira de Carvalho, L.M., Godinho, R., Santos, N., 2021. Non-invasive molecular survey of sarcoptic mange in wildlife: diagnostic performance in wolf faecal samples evaluated by multievent capture-recapture models. Pathogens,10 (2), 243-257. 

Sousa, S., Anastácio, S., Nóvoa, M., Paz-Silva, A., Madeira de Carvalho, L.M., 2021. Gastrointestinal Parasitism in Miranda Donkeys: Epidemiology and Selective Control of Strongyles Infection in the Northeast of Portugal. Animals, 11, 155-172. 



Balenghien, T., Alexander, N., Arnþórsdóttir, A.L.,  Bisia, M., Blackwell, A., Bødker, R., et al. 2020. VectorNet Data Series 3: Culicoides Abundance Distribution Models for Europe and Surrounding Regions. Journal of Open Health Data, E-ISSN: 2054-7102, 2020. 

Canhão-Dias, M., Paz-Silva, A., Madeira de Carvalho, L.M., 2020. The efficacy of predatory fungi on the control of gastrointestinal parasites in domestic and wild animals – a systematic review. Vet Parasitol, 283. 

Castro-Forero, S.P., Bulla-Castañeda, D.M., López Buitrago, H.A., Díaz Anaya, A.M., Madeira de Carvalho, L.M., Pulido-Medellín, M.O., (2020) Sarcocystis spp., a parasite with zoonotic potential. Bulg J Vet Med, 1-12. 

Figueiredo, A.M., Valente, A.M., Fonseca, C., Madeira de Carvalho, L., Torres, R.T., 2020. Endoparasite diversity of the main wild ungulates in Portugal. Wildlife Biol 2020 (1): 1-6. 

Figueiredo, AM., Valente, A.M., Barros, T., Carvalho, J., Silva, D.A.M., Fonseca, C., Madeira de Carvalho, L.M., Torres, R.T., 2020. What does the wolf eat? Assessing the diet of the endangered Iberian wolf (Canis lupus signatus) in northeast Portugal. PLoS ONE 15(3): 1-15.   

Fuehrer, H.P., Alho, A.M., Kayikci, F.N., Shahibarogh, B., Rosa, H., Tomás, J., Rocha, H., Harl, J., Madeira de Carvalho, L.M., 2020. Survey of zoonotic and non-zoonotic vector-borne pathogens in military horses in Lisbon, Portugal. Frontiers Vet Sci, Parasitol, 7, 1-7.

Gomes, J., Rocha, H., Carvalho, C., Bandeira, V., Fonseca, C., Rosalino, L.M., Cunha, M.V., 2020. Molecular detection and characterization of Leishmania infantum in free-ranging Egyptian mongoose (Herpestes ichneumon). Int J Parasitol Parasites Wildl. 2020 Feb 4;11:158-162. 

Lopes, P., Vilares, A., Caçador, T., Martins, S., Ferreira, I., Madeira de Carvalho, L.M., Gargaté, M.J., 2020. Occurrence of larval anisakids in horse mackerel (Trachurus trachurus) caught in Portuguese waters. Parasitol Research, 119, 2799–2811.

Mignotte, A., Garros, C., Gardes, L., Balenghien, T., Duhayon, M., Rakotoarivony, I., Tabourin, L., Poujol, L., Mathieu, B., et al. 2020. The tree that hides the forest: cryptic diversity and phylogenetic relationships in the Palaearctic vector Obsoletus/Scoticus Complex (Diptera: Ceratopogonidae) at the European level. Parasites & Vectors 13. 

Miró, J., Gutiérrez-Reinoso, M., Silva, J.A.D., Fernandes, C., Rebordão, M.R., Alexandre-Pires, G., Catalán, J., Ferreira-Dias, G., 2020. Collagen and Eosinophils in Jenny's Endometrium: Do They Differ With Endometrial Classification? Frontiers in Veterinary Science, 7, 631. 


Pereira, A., Teixeira, J., Sousa, S., Parreira, R., Campino, L., Meireles, J., Maia. C. (2020) Giardia duodenalis infection in dogs from the metropolitan area of Lisbon, Portugal: prevalence, genotyping and associated risk factors. J Parasit Dis.

Pessoa, V., Ramilo, D.W., da Fonseca, I.P., Ferreira, M.B., Marti, E., Tilley, P., 2020. Culicoides spp. found near Lusitano stud farms in mainland Portugal which may contribute for IBH studies. Veterinary Parasitology- Regional Studies and Reports 20. 

Rodrigues, A.V., Alexandre-Pires, G., Valerio-Bolas, A., Santos-Mateus, D., Rafael-Fernandes, M., Pereira, M.A., Ligeiro, D., Nunes, T., Alves-Azevedo, R., Santos, M., da Fonseca, I.P., Santos-Gomes, G., 2020. 3D-Hepatocyte Culture Applied to Parasitology: Immune Activation of Canine Hepatic Spheroids Exposed to Leishmania infantum. Biomedicines 8, 628. 

Santos, M.F., Alexandre-Pires, G., Pereira, M.A., Gomes, L., Rodrigues, A.V., Basso, A., Reisinho, A., Meireles, J., Santos-Gomes, G.M., da Fonseca, I.P., 2020. Immunophenotyping of Peripheral Blood, Lymph Node, and Bone Marrow T Lymphocytes During Canine Leishmaniosis and the Impact of Antileishmanial Chemotherapy. Frontiers in Veterinary Science 7. 

Tomas, A., Pereira da Fonseca, I., Valkenburg, T., Rebelo, M.T. 2020. Louse flies in Azorean and mainland populations of four Passeriformes species: A new perspective to parasite Island syndromes. Int Journal for Parasitol: Parasites and WildLife 14: 33-40.  



Bredtmann, C.M., Krücken, J., Kuzmina, T., Louro, M., Madeira De Carvalho, L.M., Samson-Himmelstjerna, G., 2019. Nuclear and mitochondrial marker sequences reveal close relationship between Coronocyclus coronatus and a potential Cylicostephanus calicatus cryptic species complex. Inf, Gen and Evol, July 2019.

Cardozo, S.V., Pereira Berto, B., Caetano, I., Tomás, A., Santos, M., Pereira da Fonseca, I., Gomes Lopes, C.W., 2019. Coccidian parasites from birds at rehabilitation centers in Portugal, with notes on Avispora bubonis in Old World. Braz. J. Vet. Parasitol. Jaboticabal, 28 (2): 187-193, 2019.

Ferreira, S., Hofer, H., Madeira de Carvalho, L., East, M.L., 2019. Gastrointestinal parasite infection in juvenile spotted hyenas and its effect on survival. Ecol and Evol. June 2019, 00:1–17. 

Figueiredo, A.M., Barros, T., Valente, A.M., Fonseca, C., Madeira de Carvalho, L.M., Torres, R.T., 2019. Prevalence of zoonotic parasites in an endangered Iberian wolf (Canis lupus signatus) population in Portugal. Mammalian Biol, 98, 154-162.

Lozano, J., Anaya, A., Palomero Salinero, A., Hoppe, E.G.L., Gomes, L., Paz-Silva, A., Rebelo, M.T., Madeira de Carvalho, L.M., 2019. Gastrointestinal Parasites of Free-Range Chickens – A Worldwide Issue. Bull UASVM Vet Med, 76 (2) 110-117. 

Noiva, R., Ruivo, P., Madeira De Carvalho, L.M., Fonseca, C.M., Fevereiro, M., Carvalho, P., Orge, L., Monteiro, M., Peleteiro, M.C., 2019. First description of a fatal equine infection with Halicephalobus gingivalis in Portugal. Relevance for public health. Vet Med & Sci, 5, 222–229. 

Pereira, M., Valério-Bolas, A., Saraiva Marques, C., Alexandre-Pires, G., Pereira da Fonseca, I., Santos-Gomes, G., 2019. Development of dog immune system: from in uterus to elderly. Vet. Sci. 6 (4): 83.

Pereira, M.A., Alexandre-Pires, G., Câmara, M., Santos, M., Martins, C., Rodrigues, A., Adriana, J., Passero, L.F.D., Pereira da Fonseca, I., Santos-Gomes, G., 2019. Canine neutrophils cooperate with macrophages in the early stages of Leishmania infantum in vitro infection. Parasite  Immunology, 41 (4): e12617. 

Ramilo, D.W.,  Monteiro, C., Carreira, M., Pereira da Fonseca, I., Cardoso, L., 2019. First report of Neotrombicula inopinata infestation in domestic cats from Portugal. Veterinary Parasitology 267: 1–3. 

Ramos, P., Carvalho, R., Rosa, F., Alexandre-Pires, G., Seixas, F., Esteves, A., Huffman, D., 2019. Huffmanela lusitana sp.n. (Nematoda: Trichosomoidida) infecting pouting, Trisopterus luscus (Teleostei: Gadidae) of the Atlantic coast of Portugal. International Journal for Parasitology: Parasites and Wildlife 9: 266-273. 

Rodrigues, A., Alexandre-Pires, G., Valério-Bolas, A., Santos-Mateus, D., Rafael-Fernandes, M., Pereira, M.A., Ligeiro, D., Nunes, T., Alves-Azevedo, R., Lopes-Ventura, S., Santos, M., Tomás, A.M., Pereira da Fonseca, I., Santos-Gomes, G., 2019. Dog hepatocytes are key effector cells in the liver innate immune response to Leishmania infantum. Parasitology 1–12.  

Ruano, Z.M., Cortinhas, A., Carolino, N., Gomes, J., Costa, M., Mateus, T.L., 2019. Gastrointestinal parasites as a possible threat to an endangered autochthonous Portuguese sheep breed. J Helminthol.  4: 94:e103. 

Santos, M.F., Alexandre-Pires, G., Pereira, M.A., Marques, C.S., Gomes, J., Correia, J., Duarte, A., Gomes, L., Rodrigues, A.V., Basso, A., Reisinho, A., Meireles, J., Santos-Mateus, D., Brito, M.T.V., Tavares, L., Santos-Gomes, G.M., da Fonseca, I.P., 2019. Meglumine antimoniate and miltefosine combined with allopurinol sustain pro-inflammatory immune environments during canine leishmaniosis treatment. Front. Vet. Sci. 6:362. 

Serrano, I., Gomes, D.,  Ramilo, D., Rebelo, M.T.,  Pereira da Fonseca, I., Moreira, A.,  Oliveira, M., 2019. An overview of zoonotic disease outbreaks and its forensic management over time. J Forensic Sci, 64 (5): 1304-1311, 2019. 

Silva, P.A., Cernea, M., Madeira de Carvalho, L.M., 2019. Anthelmintic Resistance in Equine Nematodes – A Review on the Current Situation, with Emphasis in Europe. Bull UASVM Vet Med, 76 (2) 132-142



Costa, J.L., Alho, A.M., Gomes, L., Eiras, A., Nunes, T., Vicente, G., Lopes, P., Madeira de Carvalho, L.M., 2018. Antineoplastic Chemotherapy and its Effects on the Gastrointestinal Parasitism of the Dog. The Open Parasitol J, 6, 96-105 pp. 

Figueiredo, A., Oliveira, L., Madeira De Carvalho, L.M., Fonseca, C., Torres, R.T., 2018. Helminth parasites of stone marten (Martes foina) in central Portugal. Annals Parasitol, 64(1), 65–68. 

Grilo, M.L., Gomes, L., Madeira de Carvalho, L.M., Siebert, U., Lehnert, K., 2018. Cryptosporidium sp. and Giardia sp. prevalence in marine mammal species present in German waters (North & Baltic seas). J Zoo Wildlife Med, 49 (4), 1002-1006. 

Leal, R.O., Pereira, H., Cartaxeiro, C., Delgado, E., Peleteiro, M.C.,  Pereira da Fonseca, I., 2018. Granulomatous rhinitis secondary to feline leishmaniosis: report of an unusual presentation and therapeutic complications. Journal of Feline Medicine and Surgery Open Reports 1–7. 

Santos, A.S., de Bruin, A., Veloso, A.R., Marques, C., da Fonseca, I.P., de Sousa, R., Sprong, H., Santos-Silva, M.M., 2018. Detection of Anaplasma phagocytophilum, Candidatus Neoehrlichia sp., Coxiella burnetii and Rickettsia spp. in questing ticks from a recreational park, Portugal. Ticks and Tick-borne Diseases, 9 (6): 1555-1564. 

Santos, D.W., Madeira de Carvalho, L.M., Molento, M.B., 2018. Identification of third stage larval types of cyathostomins of equids: An improved perspective. Vet Parasitol, 260, 49-52. 

Tomás, A., Rebelo, M.T., Darby, M., Pereira da Fonseca, I., 2018. An association between featherwing beetle Ptiliolum fuscum (Erichson)) (Coleoptera: Ptiliidae) and the Eurasian Griffon Gyps fulvus (Hablizi) (Accipitriformes: Accipitridae) – First report of a phoretic interaction between beetles and birds? Coleopterists Bulletin, 72 (4): 1-3, 2018. 

Tomás, A., Rebelo, M.T., Valkenburg, T., Mironov, S., Santos, M., Pereira da Fonseca, I., 2018. The first record of feather mite Alloptes (Conuralloptes) calidridis Dubinin, 1951 (Acari: Alloptidae) from the Dunlin, Calidris alpina (L., 1758) (Passeriformes: Scolopacidae) in southern of PortugalInternational Journal of Acarology, 44(6): 276-278. 

Alho, A.M., Lima, C., Colella, V., de Carvalho, L.M., Otranto, D., Cardoso, L., 2018. Awareness of zoonotic diseases and parasite control practices: a survey of dog and cat owners in Qatar. Parasite Vector 11, 133. 

Alho, A.M., Meireles, J., Schnyder, M., Cardoso, L., Belo, S., Deplazes, P., de Carvalho, L.M., 2018. Dirofilaria immitis and Angiostrongylus vasorum: The current situation of two major canine heartworms in Portugal. Vet Parasitol 252, 120-126.

Madeira de Carvalho, L.M., Alho, A.M., 2018. Teaching Veterinary Parasitology in Portugal in the 21st century - Changes, challenges and opportunities after the Bologna process. Vet Parasitol 253, 98-101.

Otero, D., Alho, A.M., Nijsse, R., Roelfsema, J., Overgaauw, P., de Carvalho, L.M., 2018. Environmental contamination with Toxocara spp. eggs in public parks and playground sandpits of Greater Lisbon, Portugal. J. Infect. Public Health 11, 94-98.

Santos, A.S., de Bruin, A., Veloso, A.R., Marques, C., da Fonseca, I.P., de Sousa, R., Sprong, H., Santos-Silva, M.M., 2018. Detection of Anaplasma phagocytophilum, Candidatus Neoehrlichia sp., Coxiella burnetii and Rickettsia spp. in questing ticks from a recreational park, Portugal. Ticks Tick-Borne Dis 9, 1555-1564.



Alexandre-Pires, G., Martins, C., Galvao, A.M., Miranda, M., Silva, O., Ligeiro, D., Nunes, T., Ferreira-Dias, G., 2017. Understanding the inguinal sinus in sheep (Ovis aries)-Morphology, secretion, and expression of progesterone, estrogens, and prolactin receptors. Int J Mol Sci 18, 1516.

Alho, A.M., Lima, C., Latrofa, M.S., Colella, V., Ravagnan, S., Capelli, G., de Carvalho, L.M., Cardoso, L., Otranto, D., 2017. Molecular detection of vector-borne pathogens in dogs and cats from Qatar. Parasite Vector 10, 298.

Alho, A.M., Marcelino, I., Colella, V., Flanagan, C., Silva, N., Correia, J.J., Latrofa, M.S., Otranto, D., de Carvalho, L.M., 2017. Dirofilaria immitis in pinnipeds and a new host record. Parasite Vector 10, 142.

Ahmed, H., Sousa, S.R., Simsek, S., Anastacio, S., Kilinc, S.G., 2017. First molecular characterization of Hypoderma actaeon in cattle and red deer (Cervus elaphus) in Portugal. Korean J. Parasitol. 55, 653-658.

Ferreira, A., Alho, A.M., Otero, D., Gomes, L., Nijsse, R., Overgaauw, P.A.M., de Carvalho, L.M., 2017. Urban dog parks as sources of canine parasites: Contamination rates and pet owner behaviours in Lisbon, Portugal. J. Environ. Public Health 2017, 5984086.

Ferreira, C., Afonso, A., Calado, M., Mauricio, I., Alho, A.M., Meireles, J., de Carvalho, L.M., Belo, S., 2017. Molecular characterization of Dirofilaria spp. circulating in Portugal. Parasite Vector 10, 250.

Giannelli, A., Capelli, G., Joachim, A., Hinney, B., Losson, B., Kirkova, Z., Rene-Martellet, M., Papadopoulos, E., Farkas, R., Napoli, E., Brianti, E., Tamponi, C., Varcasia, A., Alho, A.M., de Carvalho, L.M., Cardoso, L., Maia, C., Mircean, V., Mihalca, A.D., Miro, G., Schnyder, M., Cantacessi, C., Colella, V., Cavalera, M.A., Latrofa, M.S., Annoscia, G., Knaus, M., Halos, L., Beugnet, F., Otranto, D., 2017. Lungworms and gastrointestinal parasites of domestic cats: a European perspective. Int. J. Parasitol. 47, 517-528.

Gomes, J., Santos, M., Amaro, A., da Fonseca, I.P., Santos-Gomes, G., Inacio, J., 2017. A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle. Mol. Cell. Probes 31, 61-64.

Lempereur, L., Beck, R., Fonseca, I., Marques, C., Duarte, A., Santos, M., Zuquete, S., Gomes, J., Walder, G., Domingos, A., Antunes, S., Baneth, G., Silaghi, C., Holman, P., Zintl, A., 2017. Guidelines for the detection of Babesia and theileria parasites. Vector-Borne Zoonotic Dis. 17, 51-65.

Pereira, M., Valerio-Bolas, A., Santos-Mateus, D., Alexandre-Pires, G., Santos, M., Rodrigues, A., Rocha, H., Santos, A., Martins, C., Tomas, A., Passero, F., da Fonseca, I.P., Santos-Gomes, G., 2017. Canine neutrophils activate effector mechanisms in response to Leishmania infantum. Vet Parasitol 248, 10-20.

Pinto-Bravo, P., Galvao, A., Rebordao, M.R., Amaral, A., Ramilo, D., Silva, E., Szostek-Mioduchowska, A., Alexandre-Pires, G., da Costa, R.R., Skarzynski, D.J., Ferreira-Dias, G., 2017. Ovarian steroids, oxytocin, and tumor necrosis factor modulate equine oviduct function. Domest Anim Endocrin 61, 84-99.

Portillo, A., de Sousa, R., Santibanez, S., Duarte, A., Edouard, S., Fonseca, I.P., Marques, C., Novakova, M., Palomar, A.M., Santos, M., Silaghi, C., Tomassone, L., Zuquete, S., Oteo, J.A., 2017. Guidelines for the detection of Rickettsia spp. Vector-Borne Zoonotic Dis. 17, 23-32.

Ramilo, D.W., Nunes, T., Madeira, S., Boinas, F., da Fonseca, I.P., 2017. Geographical distribution of Culicoides (Diptera: Ceratopogonidae) in mainland Portugal: Presence/absence modelling of vector and potential vector species. Plos One 12, e0180606.

Rebordao, M.R., Alexandre-Pires, G., Carreira, M., Adriano, L., Carneiro, C., Nunes, T., Mateus, L., Ferreira-Dias, G., 2017. Bacteria causing pyometra in bitch and queen induce neutrophil extracellular traps. Vet Immunol Immunop 192, 8-12.

Rodrigues, A., Claro, M., Alexandre-Pires, G., Santos-Mateus, D., Martins, C., Valerio-Bolas, A., Rafael-Fernandes, M., Pereira, M.A., da Fonseca, I.P., Tomas, A.M., Santos-Gomes, G., 2017. Leishmania infanturn antigens modulate memory cell subsets of liver resident T lymphocyte. Immunobiology 222, 409-422.

Rodrigues, A., Santos-Mateus, D., Alexandre-Pires, G., Valerio-Bolas, A., Rafael-Fernandes, M., Pereira, M.A., Ligeiro, D., de Jesus, J., Alves-Azevedo, R., Lopes-Ventura, S., Santos, M., Tomas, A.M., da Fonseca, I.P., Santos-Gomes, G., 2017. Leishmania infantum exerts immunomodulation in canine Kupffer cells reverted by meglumine antimoniate. Comp Immunol Microb 55, 42-52.



Alho, A.M., Silva, J., Fonseca, M.J., Santos, F., Nunes, C., de Carvalho, L.M., Rodrigues, M., Cardoso, L., 2016. First report of Cytauxzoon sp infection in a domestic cat from Portugal. Parasite Vector 9, 220.

Alho, A., Mouro, S., Pissarra, H., Murta, A., Lemos, M., Gomes, L., Lima, C., de Carvalho, L., 2016. First report of Eucoleus boehmi infection in a dog from Portugal. Parasitol Res 115, 1721-1725.

Alho, A.M., Cruz, L., Coelho, A., Martinho, F., Mansinho, M., Annoscia, G., Lia, R.P., Giannelli, A., Otranto, D., de Carvalho, L.M., 2016. Aberrant laryngeal location of Onchocerca lupi in a dog. Parasitol. Int. 65, 218-220.

Alho, A.M., Fiarresga, A., Landum, M., Lima, C., Gamboa, O., Meireles, J., Sales Luis, J., Madeira de Carvalho, L., 2016. A homemade snare: An alternative method for mechanical removal of Dirofilaria immitis in dogs. Vet. Med. Int. 2016, 5780408.

Alho, A.M., Giannelli, A., Colella, V., Otranto, D., de Carvalho, L.M., Correia, J.J., 2016. Pathology in practice: Dirofilaria immitis - a silent cause of pulmonary thromboembolism and sudden death in a cat. J. Am. Vet. Med. Assoc. 249, 751-753.

Alho, A.M., Pita, J., Amaro, A., Amaro, F., Schnyder, M., Grimm, F., Custodio, A.C., Cardoso, L., Deplazes, P., de Carvalho, L.M., 2016. Seroprevalence of vector-borne pathogens and molecular detection of Borrelia afzelii in military dogs from Portugal. Parasite Vector 9, 225.

Alho, A.M., Pontes, J., Pomba, C., 2016. Guardians' knowledge and husbandry practices of feline environmental enrichment. J. Appl. Anim. Welfare Sci. 19, 115-125.

Alho, A.M., Schnyder, M., Schaper, R., Meireles, J., Belo, S., Deplazes, P., de Carvalho, L.M., 2016. Seroprevalence of circulating Angiostrongylus vasorum antigen and parasite-specific antibodies in dogs from Portugal. Parasitol Res 115, 2567-2572.

Basso, M.A., Marques, C., Santos, M., Duarte, A., Pissarra, H., Carreira, L.M., Gomes, L., Valerio-Bolas, A., Tavares, L., Santos-Gomes, G., Pereira da Fonseca, I., 2016. Successful treatment of feline leishmaniosis using a combination of allopurinol and N-methyl-glucamine antimoniate. JFMS open reports 2, 1-7.

Cardozo, S.V., Berto, B.P., Caetano, I., Maniero, V.C., da Fonseca, I.P., Lopes, C.W.G., 2016. Caryospora peneireiroi n. sp (Apicomplexa: Eimeriidae) in the common kestrel, Falco tinnunculus (Falconiformes: Falconidae), in mainland Portugal. Rev Bras Parasitol V 25, 202-206.

Couto, M., Santos, A.S., Laborda, J., Novoa, M., Ferreira, L.M., de Carvalho, L.M.M., 2016. Grazing behaviour of Miranda donkeys in a natural mountain pasture and parasitic level changes. Livest Sci 186, 16-21.

Duarte, A.M., Jenkins, T.P., Latrofa, M.S., Giannelli, A., Papadopoulos, E., de Carvalho, L.M., Nolan, M.J., Otranto, D., Cantacessi, C., 2016. Helminth infections and gut microbiota - a feline perspective. Parasite Vector 9, 625.

Figueiredo, A., Oliveira, L., Madeira de Carvalho, L., Fonseca, C., Torres, R.T., 2016. Parasite species of the endangered Iberian wolf (Canis lupus signatus) and a sympatric widespread carnivore. International journal for parasitology. Parasites and wildlife 5, 164-167.

Grilo, M.L., Vanstreels, R.E.T., Wallace, R., Garcia-Parraga, D., Braga, E.M., Chitty, J., Catao-Dias, J.L., de Carvalho, L.M.M., 2016. Malaria in penguins - current perceptions. Avian Pathol 45, 393-407.

Jacquet, S., Huber, K., Pages, N., Talavera, S., Burgin, L.E., Carpenter, S., Sanders, C., Dicko, A.H., Djerbal, M., Goffredo, M., Lhor, Y., Lucientes, J., Miranda-Chueca, M.A., Da Fonseca, I.P., Ramilo, D.W., Setier-Rio, M.L., Bouyer, J., Chevillon, C., Balenghien, T., Guis, H., Garros, C., 2016. Range expansion of the Bluetongue vector, Culicoides imicola, in continental France likely due to rare wind-transport events. Scientific Reports 6, 27247

Hernandez, J.A., Arroyo, F.L., Suarez, J., Cazapal-Monteiro, C.F., Romasanta, A., Lopez-Arellano, M.E., Pedreira, J., de Carvalho, L.M.M., Sanchez-Andrade, R., Arias, M.S., de Gives, P.M., Paz-Silva, A., 2016. Feeding horses with industrially manufactured pellets with fungal spores to promote nematode integrated control. Vet Parasitol 229, 37-44.

Matos, B., Colella, V., Alho, A.M., Otranto, D., Doyle, R., Madeira De Carvalho, L., 2016. Crenosoma vulpis infection in a four-month old puppy. Helminthologia 53, 276-280.

Soares, C.S.A., Duarte, S.C., Sousa, S.R., 2016. What do we know about feline leishmaniosis? J Feline Med Surg 18, 435-442.



Jacquet, S., Garros, C., Lombaert, E., Walton, C., Restrepo, J., Allene, X., Baldet, T., Cetre-Sossah, C., Chaskopoulou, A., Delecolle, J.C., Desvars, A., Djerbal, M., Fall, M., Gardes, L., de Garine-Wichatitsky, M., Goffredo, M., Gottlieb, Y., Gueye Fall, A., Kasina, M., Labuschagne, K., Lhor, Y., Lucientes, J., Martin, T., Mathieu, B., Miranda, M., Pages, N., Pereira da Fonseca, I., Ramilo, D.W., Segard, A., Setier-Rio, M.L., Stachurski, F., Tabbabi, A., Talla Seck, M., Venter, G., Zimba, M., Balenghien, T., Guis, H., Chevillon, C., Bouyer J., Huber, K. 2015. Colonization of the Mediterranean basin by the vector biting midge species Culicoides imicola: an old story. Mol. Ecol. 24:5707-5725. 

Ribeiro, R., Wilson, A.J., Nunes, T., Ramilo, D.W., Amador, R., Madeira, S., Baptista, F.M., Harrup, L.E., Lucientes, J., Boinas, F. 2015. Spatial and temporal distribution of Culicoides species in mainland Portugal (2005-2010). Results of the Portuguese entomological surveillance programme. PLoS One 10:e0124019

Basto, A.P., Badenes, M., Almeida, S.C.P., Martins, C., Duarte, A., Santos, D.M., Leitao, A., 2015. Immune response profile elicited by the model antigen ovalbumin expressed in fusion with the bacterial OprI lipoprotein. Mol. Immunol. 64:36-45.

Cardozo, S.V., Berto, B.P., da Fonseca, I.P., Tomas, A., Thode, F.R.P.B., Lopes, C.W.G., 2015. Characterisation of Isospora lusitanensis n. sp (Apicomplexa: Eimeriidae) from the Eurasian blackbird Turdus merula Linnaeus (Passeriformes: Turdidae) in mainland Portugal. Syst. Parasitol. 92:171-179. 

Matos, M., Alho, A.M., Owen, S.P., Nunes, T., de Carvalho, L.M., 2015. Parasite control practices and public perception of parasitic diseases: A survey of dog and cat owners. Prev. Vet. Med. 122: 174-180.

Cernea, M., Cristina, R.T., Stefanut, L.C., de Carvalho, L.M.M., Taulescu, M.A., Cozma, V., 2015. Screening for anthelmintic resistance in equid strongyles (Nematoda) in Romania. Folia Parasitol. (Praha) 62:023.

Marques, C.S., Passero, L.F.D., Vale-Gato, I., Rodrigues, A., Rodrigues, O.R., Martins, C., Correia, I., Tomas, A.M., Alexandre-Pires, G., Ferronha, M.H., Santos-Gomes, G.M., 2015. New insights into neutrophil and Leishmania infantum in vitro immune interactions. Comp. Immunol. Microbiol. .Infectious Dis. 40:19-29.

Sanchis, J., Hillyer, G.V., de Carvalho, L.M.M., Macchi, M.I., Gomes, C., Maldini, G., Stilwell, G., Venzal, J.M., Paz-Silva, A., Sanchez-Andrade, R., Arias, M.S., 2015. Risk of exposure to Fasciola hepatica in cattle under extensive management from Uruguay and Portugal deteimined by ELISA using a recombinant antigen. Arch. Med. Vet. 47: 201-208. doi:


Waap, H., Nunes, T., Cortes, H., Leitão, A., Vaz, Y. 2014. Prevalence and geographic distribution of Besnoitia besnoiti infection in cattle herds in Portugal. Parasitol. Res. 113:3703-3711.

Basto, A.P., Leitão, A. 2014. Targeting TLR2 for vaccine development. J. Immunol. Res., 2014:619410.

Basto, A.P., Morais, J., Marcelino, E., Leitão, A., Santos, D.M. 2014. An efficient depyrogenation method for recombinant bacterial outer membrane lipoproteins. Protein Expr. Purif., 98: 10-17.

Bourdeau, P., Saridomichelakis, M.N., Oliva, A.O.G., Kotnik, T., Gálvez, R., Manzillo, V.F., Koutinas, A.F., Fonseca, I.P., Miró, G. 2014. Management of canine leishmaniosis in endemic SW European regions: a questionnaire-based multinational survey. Parasit. Vectors 7:110.

Cardoso, R., Nolasco, S., Gonçalves, J., Cortes, H.C., Leitão, A., Soares, H. 2014. Besnoitia besnoiti and Toxoplasma gondii: two apicomplexan strategies to manipulate the host cell centrosome and Golgi apparatus. Parasitology 141:1436-1454.

Cortes, H., Leitão, A., Gottstein, B., Hemphill, A. 2014. A review on bovine besnoitiosis: a disease with economic impact in herd health management, caused by Besnoitia besnoiti (Franco and Borges, 1916). Parasitology 141:1406-1417.

Hemphill, A., Leitao, A. 2014. ApiCOWplexa 2013 – 2nd International Meeting on Apicomplexan Parasites in Farm Animals. Special issue preface. Parasitology 141: 1355-1358.

Landum, M., Ferreira, C., Calado, M., Alho, A., Maurício, I., Meireles, J., Madeira de Carvalho, L., Cunha, C., Belo, S. 2014. Detection of Wolbachia in Dirofilaria infected dogs in Portugal. Vet. Parasitol. 204:4017-410.

Alexandre-Pires, G., Martins, C., Galvao, A.M., Correia, M., Ramilo, D., Quaresma, M., Ligeiro, D., Nunes, T., Caldeira, R.M., Ferreira-Dias, G., 2014. Morphological aspects and expression of estrogen and progesterone receptors in the interdigital sinus in cyclic ewes. Microsc. Res. Techn. 77:313-325.

Alho, A.M., Landum, M., Ferreira, C., Meireles, J., Goncalves, L., de Carvalho, L.M., Belo, S., 2014. Prevalence and seasonal variations of canine dirofilariosis in Portugal. Vet. Parasitol. 206:99-105. 

Santos-Gomes, G.M., Rodrigues, A., Teixeira, F., Carreira, J., Alexandre-Pires, G., Carvalho, S., Santos-Mateus, D., Martins, C., Vale-Gato, I., Marques, C., Tomas, A.M., 2014. Immunization with the Leishmania infantum recombinant cyclophilin protein 1 confers partial protection to subsequent parasite infection and generates specific memory T cells. Vaccine 32:1247-1253. 



Arias, M., Cazapal-Monteiro, C., Valderrábano, E., Miguélez, S., Rois, J.L., López-Arellano, M.E., Madeira de Carvalho, L., Gives, P.M., Sánchez-Andrade, R., Paz-Silva, A. 2013. A preliminary study of the biological control of strongyles affecting equids in a zoological park. J. Equine Vet. Sci. 33:1115-1120.

Cunha, R.C., Andreotti, R., Garcia, M.V., Aguirre, A.D.R., Leitão, A. 2013. Calculation of the efficacy of vaccines against tick infestations on cattle. R. Bras. Parasitol. Vet. 22: 571-578.

Guerra, D., Armua-Fernandez, M.T., Silva, M., Bravo, I., Santos, N., Deplazes, P., Madeira de Carvalho, L.M. 2013. Taeniid species of the Iberian wolf (Canis lupus signatus) in Portugal with special focus on Echinococcus spp. Int. J. Parasitol. Parasites Wildl. 2: 50-53.

Helhazar, M., Leitão, J., Duarte, A., Tavares, L., Fonseca, I.P. 2013. Natural infection of synathropic rodent species Mus musculus and Rattus rattus by Leishmania infantum in 2 Sesimbra and Sintra – Portugal. Parasit. Vectors 6: 88.

Hemphill, A., Leitao, A., Cooke, B.M. 2013. International Meeting on Apicomplexan Parasites in Farm Animals (ApiCOWplexa) 2012. Special issue editorial. Int. J. Parasitol. 43: 105-106.

Mottola, C., Freitas, F.B., Simões, M., Martins, C., Leitão, A., Ferreira, F. 2013. In vitro antiviral activity of fluoroquinolones against African swine fever virus. Vet. Microb. 165: 86-94.

Ramilo, D., Garros, C., Mathieu, B., Benedet, C., Allene, X., Silva, E., Alexandre-Pires, G., Fonseca, I.P., Carpenter, S., Radorva, J., Delecolle, J.-C. 2013. Description of Culicoides paradoxalis sp. nov. from France and Portugal (Diptera: Ceratopogonidae). Zootaxa 3745: 243-256.

Soares, C., Sousa, S.R., Anastácio, S., Matias, M.G., Marquês, I., Mascarenhas, S., Vieira, M.J., Madeira De Carvalho, L.M., Otranto, D. 2013. Feline thelaziosis caused by Thelazia callipaeda in Portugal. Vet. Parasitol., 196: 528-531.



Basto, A.P., Piedade, J., Ramalho, R., Alves, S., Soares, H., Cornelis, P., Martins, C., Leitão A. 2012. A new cloning system based on the OprI lipoprotein for the production of recombinant bacterial cell wall-derived immunogenic formulations. J. Biotechnol. 157: 50-63.

Cardoso, L., Mendão, C., Madeira de Carvalho, L.M. 2012. Prevalence of Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp. and Leishmania infantum in apparently healthy and CVBD-suspect dogs in Portugal – a national serological study. Parasit. Vectors 5: 62.

Cufos, N., Jabbar, A., M. de Carvalho, L., Gasser, R.B. 2012. Mutation scanning based analysis of Theileria orientalis populations in cattle following an outbreak. Electrophoresis 33: 2036-2040.

Diaz, S., Rodrigues, A., Martins, C., Cartaxeiro, C., Silva, M.J., Villa de Brito, T., Alexandre-Pires, G., Fonseca, I.P., Santos-Gomes, G. 2012. Canine leishmaniosis. Modulation of macrophage/lymphocyte interactions by L. infantum. Vet. Parasitol. 189: 137– 144.

Ramilo, D., Diaz, S., Fonseca, I.P., Delécolle, J.-C., Wilson, A., Meireles, J., Lucientes, J., Ribeiro, R., Boinas, F. 2012. First report of 13 species of Culicoides (Diptera:Ceratopogonidae) in mainland Portugal and Azores by morphological and molecular characterization. PLoS ONE 7: e34896.

Waap, H., Cardoso, R., Leitão, A., Nunes, T., Vilares, A., Gargaté, M.J., Meireles, J., Cortes, H., Ângelo, H. 2012. In vitro isolation and seroprevalence of Toxoplasma gondii in stray cats and pigeons in Lisbon, Portugal. Vet. Parasitol. 187: 542-547.



Barbosa, M. A. G., Alexandre-Pires, G., Soares-Clemente, M., Marques, C., Rodrigues, O.R., Villa de Brito, T., Fonseca, I.P., Alves, L. C., Santos-Gomes, G. M. 2011. Cytokine gene expression in the tissues of dogs Infected by Leishmania infantum. J. Comp. Pathol. 145:336-344.

Marcelino, E., Martins, T.M., Morais, J.B., Nolasco, S., Cortes, H., Hemphill, A., Leitão, A., Novo, C. 2011. Besnoitia besnoiti protein disulfide isomerase (BbPDI): molecular characterization, expression and in silico modelling. Exp. Parasitol. 129: 164-74.

Waap, H., Cardoso, R., Marcelino, E., Malta, J., Cortes, H., Leitão, A. 2011. A modified agglutination test for the diagnosis of Besnoitia besnoiti infection. Vet. Parasitol. 178: 217-222.



Alexandre-Pires, G., Villa de Brito, M.T., Algueró, C., Martins, C., Rodrigues, O.R., Fonseca, I.P., Santos-Gomes, G. 2010. Canine leishmaniosis – Characterization of lymphocyte phenotype profile in different sites of infection of symptomatic, asymptomatic and treated dogs. Vet. Immunol. Immunopathol. 137:275-283.

Branco, S., Orvalho, J., Leitão, A., Pereira, I., Malta, M., Mariano, I., Carvalho, T., Baptista, R., Shiels, B.R., Peleteiro, M.C. 2010. Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation. J. Vet. Sci. 11: 27-34.

Duarte, A., Castro, I., Pereira da Fonseca, I.M., Almeida, V., Madeira de Carvalho, L.M., Meireles, J., Fazendeiro, M.I., Tavares, L., Vaz, Y. 2010. Survey of infectious and parasitic diseases in stray cats at the Lisbon metropolitan area, Portugal. J. Feline Med. Surg. 12: 441-446.

Fazenda, I.P., Gasser, R.B., Carvalho, L.M., Beveridge, I. 2010. Resurrection and redescription of Globocephaloides wallabiae Johnston et Mawson, 1939 (Nematoda, Trichostrongyloidea) from macropodid marsupials in north-eastern Australia. Acta Parasitologica, 55: 138-143.



Gallardo, C., Reis, A.L., Kalema-Zikusoka, G., Malta, J., Soler, A., Blanco, E., Parkhouse, R.M., Leitão, A. 2009. Recombinant antigen targets for serodiagnosis of African swine fever. Clin. Vaccine Immunol. 16:1012-1020.

Fazenda, I.P, Beveridge, I., Chilton, N.B., Jex, A.R., Pangasa, A., Campbell, B.E., Florence Huby-Chilton, F., Madeira de Carvalho, L., Gasser, R.B. 2009. Analysis of genetic variation in Globocephaloides populations from macropodid marsupials using a mutation scanning-based approach. Electrophoresis 30:2758-2764.

Makoschey, B., Klee, W., Decaro, N., Bridger, J., Smith, D., Daugschies, A., Millemann, Y., Liebler-Tenorio, E., Snodgrass, D., Claerebout, E., Bendali, F., Van De Ven, J., Garcia, A., Illek, J., Kaske, M., Cutler, K., Vincente Gonzales, J., Madeira Carvalho, L.M., Crouch, C., Thiry, E. 2009. Neonatal health in calves – Comprehensive solutions for complex enteric disorders. Berl. Münch. Tierärztl. Wschr. 122: 404-408.

Portugal, R., Leitão, A., Martins, C. 2009. Characterization of African swine fever virus IAP homologue expression in porcine macrophages infected with different virulence isolates. Vet. Microbiol. 139: 140–146.

Portugal, R., Leitão, A., Martins, C. 2009. Apoptosis in porcine macrophages infected in vitro with African swine fever virus (ASFV) strains with different virulence. Arch. Virol. 154: 1441-1450.


Book chapters

Madeira de Carvalho, L. M., Bernardo, F.A., Paz-Silva, A. 2012. The role of fungi in the control of animal parasites – Classification, mode of action and practical applications. pp. 271-308. In: Fungi: Types, Environmental Impact and Role in Disease. Eds. A. Paz-Silva, M.S. Arias Vázquez. Nova Science Publishers. New York, USA; ISBN: 978-1-61942-671-9.

Madeira de Carvalho, L. M., Sousa, S., Cernea, M., Cernea, L.C., Arias, M., Paz-Silva, A. 2014. Strongyles shed in faeces as a means of monitoring the parasite scenario in horse stud farms. pp. 93-125. In: Horses: Breeding, Health Disorders and Effects on Performance & Behavior. Eds. A. Paz-Silva, M.S. Arias, R. Sanchéz-Andrade. Nova Science Publishers, New York, USA, ISBN: 978-1-63117-560-2.

Sanchís Polto, J., Madeira de Carvalho, L.M., Bonilla, R., Duque de Araújo, A.M., Arroyo, F., Suaréz, J., Solari, M.A., Romero, J.A., Sanchéz-Andrade, R. 2014. Horse handling conditions and emergence of neglected infections: Fasciolosis. pp. 127-143. In: Horses: Breeding, Health Disorders and Effects on Performance & Behavior. Eds. A. Paz-Silva, M.S. Arias, R. Sanchéz-Andrade. Nova Science Publishers, New York, USA, ISBN: 978-1-63117-560-2.

Santos-Gomes, G., Alexandre-Pires, G., Meireles, J., Correia, J., Villa de Brito, T., Pereira da Fonseca, I. 2011. Canine Leishmaniosis: An expanding disease (Chapter 4). In: Dogs: Biology, Behavior and Health Disorders. Ed. V.M. De Giovine. Pp. 111-126. Nova Science Publishers, New York, USA. ISBN 978-1-61209-653-7

Alexandre-Pires, G., Ramilo, D., Diaz, S., Meireles, J., Boinas, F., Pereira da Fonseca, I. 2010. Investigating morphological structures of Culicoides from Obsoletus complex by using scanning electron microscopy and composed optical microscopy. In: Microscopy: Science, Technology, Applications and Education, Ed. A. Méndez-Vilas, J.D. Álvarez, Formatex Research Center, Vol. II (13): 792-802, ISBN 978-84-614-6190-5.