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Animal Nutrition and Biotechnology

SCI papers since 2009

Publications of the "Animal Nutrition and Biotechnology Lab" team members

2021


Ahmed, J., Kumar, K., Sharma, K., Fontes, C.M.G.A., Goyal, A., 2021. Computational and SAXS-based structure insights of pectin acetyl esterase ( Ct Pae12B) of family 12 carbohydrate esterase from Clostridium thermocellum ATCC 27405. J Biomol Struct Dyn 1–18.

Costa, M., Pio, L., Bule, P., Cardoso, V., Alfaia, C.M., Coelho, D., Brás, J., Fontes, C.M.G.A., Prates, J.A.M., 2021. An individual alginate lyase is effective in the disruption of Laminaria digitata recalcitrant cell wall. Sci Rep 11, 9706.

Duarte, M., Viegas, A., Alves, V.D., Prates, J.A.M., Ferreira, L.M.A., Najmudin, S., Cabrita, E.J., Carvalho, A.L., Fontes, C.M.G.A., Bule, P., 2021. A dual cohesin–dockerin complex binding mode in Bacteroides cellulosolvens contributes to the size and complexity of its cellulosome. J Biol Chem 296, 100552.

Alfaia, C.M., Pestana, J.M., Rodrigues, M., Coelho, D., Aires, M.J., Ribeiro, D.M., Major, V.T., Martins, C.F., Santos, H., Lopes, P.A., Lemos, J.P.C., Fontes, C.M.G.A., Lordelo, M.M., Prates, J.A.M., 2021. Influence of dietary Chlorella vulgaris and carbohydrate-active enzymes on growth performance, meat quality and lipid composition of broiler chickens. Poultry Sci 100, 926–937.

Sharma, K., Fontes, C.M.G.A., Najmudin, S., Goyal, A., 2021. Small angle X-ray scattering based structure, modeling and molecular dynamics analyses of family 43 glycoside hydrolase α-L-arabinofuranosidase from Clostridium thermocellum. J Biomol Struct Dyn 39, 209–218.

 

2020


Pestana, J.M., Puerta, B., Santos, H., Madeira, M.S., Alfaia, C.M., Lopes, P.A., Pinto, R.M.A., Lemos, J.P.C., Fontes, C.M.G.A., Lordelo, M.M., Prates, J.A.M., 2020. Impact of dietary incorporation of Spirulina (Arthrospira platensis) and exogenous enzymes on broiler performance, carcass traits, and meat quality. Poultry Sci 99, 4100.

Coelho, D., Lopes, P.A., Cardoso, V., Ponte, P., Bras, J., Madeira, M.S., Alfaia, C.M., Bandarra, N.M., Fontes, C.M.G.A., Prates, J.A.M., 2020. A two-enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets. J Anim Physiol An N 104, 310-321.

Coelho, D., Pestana, J., Almeida, J.M., Alfaia, C.M., Fontes, C.M.G.A., Moreira, O., Prates, J.A.M., 2020. A High Dietary Incorporation Level of Chlorella vulgaris Improves the Nutritional Value of Pork Fat without Impairing the Performance of Finishing Pigs. Animals 10.

Reynaud, S., Ciolek, J., Degueldre, M., Saez, N.J., Sequeira, A.F., Duhoo, Y., Bras, J.L.A., Meudal, H., Diez, M.C., Pedrosa, V.F., Verdenaud, M., Boeri, J., Ramos, O.P., Ducancel, F., Vanden Driessche, M., Fourmy, R., Violette, A., Upert, G., Mourier, G., Beck-Sickinger, A.G., Morl, K., Landon, C., Fontes, C.M.G.A., Herraiz, R.M., de la Vega, R.C.R., Peigneur, S., Tytgat, J., Quinton, L., De Pauw, E., Vincentelli, R., Servent, D., Gilles, N., 2020. A Venomics Approach Coupled to High-Throughput Toxin Production Strategies Identifies the First Venom-Derived Melanocortin Receptor Agonists. J Med Chem 63, 8250-8264.

Strazzulli, A., Cobucci-Ponzano, B., Iacono, R., Giglio, R., Maurelli, L., Curci, N., Schiano-di-Cola, C., Santangelo, A., Contursi, P., Lombard, V., Henrissat, B., Lauro, F.M., Fontes, C.M.G.A., Moracci, M., 2020. Discovery of hyperstable carbohydrate-active enzymes through metagenomics of extreme environments. Febs J 287, 1116-1137.

 

2019 


Ribeiro, D.O., Viegas, A., Pires, V.M.R., Medeiros-Silva, J., Bule, P., Chai, W.G., Marcelo, F., Fontes, C.M.G.A., Cabrita, E.J., Palma, A.S., Carvalho, A.L., 2019. Molecular basis for the preferential recognition of beta 1,3-1,4-glucans by the family 11 carbohydrate-binding module from Clostridium thermocellum. Febs J 287, 2723-2743.

Bule, P., Chuzel, L., Blagova, E., Wu, L., Gray, M.A., Henrissat, B., Rapp, E., Bertozzi, C.R., Taron, C.H., Davies, G.J., 2019. Inverting family GH156 sialidases define an unusual catalytic motif for glycosidase action. Nat Commun 10, 4816.

Chong, S.L., Cardoso, V., Bras, J.L.A., Gomes, M.Z.d.V., Fontes, C.M.G.A., Olsson, L., 2019. Immobilization of bacterial feruloyl esterase on mesoporous silica particles and enhancement of synthetic activity by hydrophobic-modified surface. Bioresource Technol 293.

Goyal, D., Kumar, K., Centeno, M.S.J., Thakur, A., Pires, V.M.R., Bule, P., Fontes, C.M.G.A., Goyal, A., 2019. Molecular Cloning, Expression and Biochemical Characterization of a Family 5 Glycoside Hydrolase First Endo-Mannanase (RfGH5_7) from Ruminococcus flavefaciens FD-1 v3. Mol Biotechnol 61, 826-835.

Sharma, K., Fontes, C.M.G.A., Najmudin, S., Goyal, A., 2019. Molecular organization and protein stability of the Clostridium thermocellum glucuronoxylan endo-beta-1,4-xylanase of family 30 glycoside hydrolase in solution. J Struct Biol 206, 335-344.

 

2018


Bule, P., Cameron, K., Prates, J.A.M., Ferreira, L.M.A., Smith, S.P., Gilbert, H.J., Bayer, E.A., Najmudin, S., Fontes, C., Alves, V.D., 2018. Structure-function analyses generate novel specificities to assemble the components of multienzyme bacterial cellulosome complexes. J Biol Chem 293, 4201-4212.

Bule, P., Pires, V.M.R., Alves, V.D., Carvalho, A.L., Prates, J., Ferreira, L.M.A., Smith, S.P., Gilbert, H.J., Noach, I., Bayer, E.A., Najmudin, S., Fontes, C., 2018. Higher order scaffoldin assembly in Ruminococcus flavefaciens cellulosome is coordinated by a discrete cohesin-dockerin interactionSci Rep 8, 6987.

Bule, P., Pires, V.M.R., Fontes, C.M.G.A., Alves, V.D., 2018. Cellulosome assembly: paradigms are meant to be broken! Curr Opin Struc Biol 49, 154-161.

Cardoso, V., Fernandes, E.A., Santos, H.M.M., Macas, B., Lordelo, M.M., da Gama, L.T., Ferreira, L.M.A., Fontes, C., Ribeiro, T., 2018. Variation in levels of non-starch polysaccharides and endogenous endo-1,4--xylanases affects the nutritive value of wheat for poultryBrit Poultry Sci 59, 218-226.

Kryshtafovych, A., Albrecht, R., Basle, A., Bule, P., Caputo, A.T., Carvalho, A.L., Chao, K.L., Diskin, R., Fidelis, K., Fontes, C., Fredslund, F., Gilbert, H.J., Goulding, C.W., Hartmann, M.D., Hayes, C.S., Herzberg, O., Hill, J.C., Joachimiak, A., Kohring, G.W., Koning, R.I., Lo Leggio, L., Mangiagalli, M., Michalska, K., Moult, J., Najmudin, S., Nardini, M., Nardone, V., Ndeh, D., Nguyen, T.H., Pintacuda, G., Postel, S., van Raaij, M.J., Roversi, P., Shimon, A., Singh, A.K., Sundberg, E.J., Tars, K., Zitzmann, N., Schwede, T., 2018. Target highlights from the first post-PSI CASP experiment (CASP12, May-August 2016)Proteins 86, 27-50.

Kumar, K., Correia, M.A.S., Pires, V.M.R., Dhillon, A., Sharma, K., Rajulapati, V., Fontes, C.M.G.A., Carvalho, A.L., Goyal, A., 2018. Novel insights into the degradation of beta-1,3-glucans by the cellulosome of Clostridium thermocellum revealed by structure and function studies of a family 81 glycoside hydrolaseInt. J. Biol. Macromol. 117, 890-901.

Dhifaoui, S., Nasri, S., Gontard, G., Ghosh, A.C., Garcia, Y., Bonifàcio, C., Najmudin, S., Marvaud, V., Nasri, H., 2018. Synthesis, Mössbauer, cyclic voltammetry, magnetic properties and molecular structures of the low-spin iron(III) bis(pyrazine) complexes with the para-fluoro and para-chloro substituted meso-tetraphenylporphyrin. Inorg Chim Acta 477, 114–121.

Ribeiro, T., Cardoso, V., Ferreira, L.M.A., Lordelo, M.M.S., Coelho, E., Moreira, A.S.P., Domingues, M.R.M., Coimbra, M.A., Bedford, M.R., Fontes, C.M.G.A., 2018. Xylo-oligosaccharides display a prebiotic activity when used to supplement wheat or corn-based diets for broilers. Poultry Sci 97, 4330-4341

 

2017


Bule, P., Alves, V.D., Israeli-Ruimy, V., Carvalho, A.L., Ferreira, L.M.A., Smith, S.P., Gilbert, H.J., Najmudin, S., Bayer, E.A., Fontes, C.M.G.A., 2017. Assembly of Rumincoccus flavefaciens cellulosome revealed by structures of two cohesin-dockerin complexes. Sci Rep 7, 759

Israeli-Ruimy, V., Bule, P., Jindou, S., Dassa, B., Morais, S., Borovok, I., Barak, Y., Slutzki, M., Hamberg, Y., Cardoso, V., Alves, V.D., Najmudin, S., White, B.A., Flint, H.J., Gilbert, H.J., Lamed, R., Fontes, C.M.G.A., Bayer, E.A., 2017. Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family-related protein-protein interactions. Scientific Reports 7.

Pires, V.M.R., Pereira, P.M.M., Bras, J.L.A., Correia, M., Cardoso, V., Bule, P., Alves, V.D., Najmudin, S., Venditto, I., Ferreira, L.M.A., Romao, M.J., Carvalho, A.L., Fontes, C.M.G.A., Prazeres, D.M., 2017. Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure of Spirochaeta thermophila StCBM64C. J. Biol. Chem. 292, 4847-4860.

Sequeira, A.F., Turchetto, J., Saez, N.J., Peysson, F., Ramond, L., Duhoo, Y., Blemont, M., Fernandes, V.O., Gama, L.T., Ferreira, L.M.A., Guerreiro, C.I.P.I., Gilles, N., Darbon, H., Fontes, C.M.G.A., Vincentelli, R., 2017. Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli. Microbial Cell Factories 16, 4.

Turchetto, J., Sequeira, A.F., Ramond, L., Peysson, F., Bras, J.L.A., Saez, N.J., Duhoo, Y., Blemont, M., Guerreiro, C.I.P.D., Quinton, L., De Pauw, E., Gilles, N., Darbon, H., Fontes, C.M.G.A., Vincentelli, R., 2017. High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery. Microbial Cell Factories 16, 6.

Cantante, C., Lourenco, S., Morais, M., Leandro, J., Gano, L., Silva, N., Leandro, P., Serrano, M., Henriques, A.O., Andre, A., Cunha-Santos, C., Fontes, C., Correia, J.D.G., Aires-Da-Silva, F., Goncalves, J., 2017. Albumin-binding domain from Streptococcus zooepidemicus protein Zag as a novel strategy to improve the half-life of therapeutic proteins. J Biotechnol 253, 23-33.

Lee, S.A., Apajalahti, J., Vienola, K., Gonzalez-Ortiz, G., Fontes, C., Bedford, M.R., 2017. Age and dietary xylanase supplementation affect ileal sugar residues and short chain fatty acid concentration in the ileum and caecum of broiler chickens. Anim Feed Sci Tech 234, 29-42.

 

2016


Bras, J.L.A., Pinheiro, B.A., Cameron, K., Cuskin, F., Viegas, A., Najmudin, S., Bule, P., Pires, V.M.R., Romao, M.J., Bayer, E.A., Spencer, H.L., Smith, S., Gilbert, H.J., Alves, V.D., Carvalho, A.L., Fontes, C.M.G.A., 2016. Diverse specificity of cellulosome attachment to the bacterial cell surface. Sci Rep 6, 12.

Bule, P., Alves, V.D., Leitao, A., Ferreira, L.M.A., Bayer, E.A., Smith, S.P., Gilbert, H.J., Najmudin, S., Fontes, C.M.G.A., 2016. Single Binding Mode Integration of Hemicellulose-degrading Enzymes via Adaptor Scaffoldins in Ruminococcus flavefaciens Cellulosome. J. Biol. Chem. 291, 26658-26669.

Dhillon, A., Fernandes, V.O., Dias, F.M.V., Prates, J.A.M., Ferreira, L.M.A., Fontes, C.M.G.A., Centeno, M.S.J., Goyal, A., 2016. A New Member of Family 11 Polysaccharide Lyase, Rhamnogalacturonan Lyase (CtRGLf) from Clostridium thermocellum. Molecular Biotechnology 58, 232-240.

Freire, F., Verma, A., Bule, P., Alves, V.D., Fontes, C.M.G.A., Goyal, A., Najmudin, S., 2016. Conservation in the mechanism of glucuronoxylan hydrolysis revealed by the structure of glucuronoxylan xylanohydrolase (CtXyn30A) from Clostridium thermocellum. Acta Crystallographica Section D-Structural Biology 72, 1162-1173.

Goyal, A., Ahmed, S., Sharma, K., Gupta, V., Bule, P., Alves, V.D., Fontes, C.M.G.A., Najmudin, S., 2016. Molecular determinants of substrate specificity revealed by the structure of Clostridium thermocellum arabinofuranosidase 43A from glycosyl hydrolase family 43 subfamily 16. Acta Crystallographica Section D-Structural Biology 72, 1281-1289.

Labourel, A., Crouch, L.I., Bras, J.L.A., Jackson, A., Rogowski, A., Gray, J., Yadav, M.P., Henrissat, B., Fontes, C.M.G.A., Gilbert, H.J., Najmudin, S., Basle, A., Cuskin, F., 2016. The Mechanism by Which Arabinoxylanases Can Recognize Highly Decorated Xylans. J. Biol. Chem. 291, 22149-22159.

Nash, M.A., Smith, S.P., Fontes, C.M.G.A., Bayer, E.A., 2016. Single versus dual-binding conformations in cellulosomal cohesin-dockerin complexes. Curr. Opin. Struc. Biol. 40, 89-96.

Sequeira, A.F., Bras, J.L.A., Guerreiro, C.I.P.D., Vincentelli, R., Fontes, C.M.G.A., 2016. Development of a gene synthesis platform for the efficient large scale production of small genes encoding animal toxins. Bmc Biotechnology 16.

Sequeira, A.F., Guerreiro, C.I.P.D., Vincentelli, R., Fontes, C.M.G.A., 2016. T7 Endonuclease I Mediates Error Correction in Artificial Gene Synthesis. Molecular Biotechnology 58, 573-584.

Soares, M., Ribeiro, R., Najmudin, S., Gameiro, A., Rodrigues, R., Cardoso, F., Ferreira, F., 2016. Serum HER2 levels are increased in cats with mammary carcinomas and predict tissue HER2 status. Oncotarget 7, 17314-17326.

Venditto, I., Luis, A.S., Rydahl, M., Schuckel, J., Fernandes, V.O., Vidal-Melgosa, S., Bule, P., Goyal, A., Pires, V.M.R., Dourado, C.G., Ferreira, L.M.A., Coutinho, P.M., Henrissat, B., Knox, J.P., Basle, A., Najmudin, S., Gilbert, H.J., Willats, W.G.T., Fontes, C.M.G.A., 2016. Complexity of the Ruminococcus flavefaciens cellulosome reflects an expansion in glycan recognition. Proc. Natl. Acad. Sci. U. S. A. 113, 7136-7141.

Fernandes, V.O., Costa, M., Ribeiro, T., Serrano, L., Cardoso, V., Santos, H., Lordelo, M., Ferreira, L.M.A., Fontes, C.M.G.A. 2016. 1,3-1,4-beta-Glucanases and not 1,4-beta-glucanases improve the nutritive value of barley-based diets for broilers. Anim. Feed Sci. Technol. 211:153-163.

Mesquita, P., Garcia, V., Marques, M.R., Santos-Silva, F., Oliveira Sousa. M.C., Carolino, I., Pimenta, J., Fontes, C.M., Horta, A.E., Prates, J.A., Pereira R.M. 2016. The prion-related protein (testis-specific) gene (PRNT) is highly polymorphic in Portuguese sheep. Anim Genet. 47:128-132.

 

2015


Palma, A.S., Liu, Y., Zhang, H., Zhang, Y., McCleary, B.V., Yu, G., Huang, Q., Guidolin, L.S., Ciocchini, A.E., Torosantucci, A., Wang, D., Carvalho, A.L., Fontes, C.M.G.A., Mulloy, B., Childs, R.A., Feizi, T., Chai, W. 2015. Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry. Mol. Cell. Proteomics 14:974-988. 

Verma, A.K., Bule, P., Ribeiro, T., Brás, J.L.A., Mukherjee, J., Gupta, M.N., Fontes, C.M.G.A., Goyal, A. 2015. The family 6 Carbohydrate Binding Module (CtCBM6) of glucuronoxylanase (CtXynGH30) of Clostridium thermocellum binds decorated and undecorated xylans through cleft A. Arch. Biochem. Biophys. 575:8-21.

Chakraborty, S., Fernandes, V.O., Dias, F.M.V., Prates, J.A.M., Ferreira, L.M.A., Fontes, C.M.G.A., Goyal, A., Centeno, M.S.J. 2015. Role of pectinolytic enzymes identified in Clostridium thermocellum cellulosome. Plos One 10:e0116787. 

Pires, A.J., Ribeiro, T., Thompson, A.,Venditto, I., Fernandes, V.O., Bule, P., Santos, H., Alves, V.D., Pires, V., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S. 2015. Purification and crystallographic studies of a putative carbohydrate-binding module from the Ruminococcus flavefaciens FD-1 endoglucanase Cel5A. Acta Crystallogr. F Struct. Biol. Commun. 71:958-961. 

Venditto, I., Najnnudin, S., Luis, A.S., Ferreira, L.M.A., Sakka, K., Knox, J.P., Gilbert, H.J., Fontes, C.M.G.A. 2015. Family 46 carbohydrate-binding modules contribute to the enzymatic hydrolysis of xyloglucan and beta-1,3-1,4-glucans through distinct mechanisms. J. Biol. Chem. 290:10572-10586.

Venditto, I., Bule, P., Thompson, A., Sanchez-Weatherby, J., Sandy, J., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S. 2015. Expression, purification, crystallization and preliminary X-ray analysis of CttA, a putative cellulose-binding protein from Ruminococcus flavefaciens. Acta Crystallogr. F Struct. Biol. Commun. 71:784-789. 

Venditto, I., Goyal, A., Thompson, A., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S. 2015. Crystallization and preliminary crystallographic studies of a novel noncatalytic carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome. Acta Crystallogr. F Struct. Biol. Commun. 71:45-48.

Cameron, K., Weinstein, J. Y., Zhivin, O., Bule, P., Fleishman, S.J., Alves, V.D., Gilbert, H.J., Ferreira, L.M.A., Fontes, C.M.G.A., Bayer, E.A., Najmudin, S. 2015. Combined crystal structure of a type I cohesin - Mutation and affinity binding studies reveal structural determinants of cohesin-dockerin specificities. J. Biol. Chem. 290:16215-16225. 

Cameron, K., Najmudin, S., Alves, V.D., Bayer, E.A., Smith, S.P., Bule, P., Waller, H., Ferreira, L.M.A., Gilbert, H.J., Fontes, C.M.G.A. 2015. Cell-surface attachment of bacterial multienzyme complexes involves highly dynamic protein-protein anchors. J. Biol. Chem. 290:13578-13590.

Denden, Z., Ezzayani, K., Saint-Aman, E., Loiseau, F., Najmudin, S., Bonifácio, C., Daran, J.C., Nasri, H. 2015. Insights on the UV/Vis, fluorescence, and cyclic voltammetry properties and the molecular structures of Zn-II tetraphenylporphyrin complexes with pseudohalide axial azido, cyanato-N, thiocyanato-N, and cyanido ligands. Eur. J. Inorg. Chem. 2015:2596-2610.
 

2014


Cardoso, V., Ferreira, A.P., Costa, M., Ponte, P.I.P., Falcao, L., Freire, J.P., Lordelo, M.M., Ferreira, L.M.A., Fontes, C.M.G.A., Ribeiro, T., 2014. Temporal restriction of enzyme supplementation in barley-based diets has no effect in broiler performance. Anim. Feed Sci. Technol. 198:186-195.

Das, S.P., Ravindran, R., Ghosh, A., Deka, D., Das, D., Jawed, M., Fontes, C.M.G.A., Goyal, A., 2014. Efficient pretreatment for bioethanol production from water hyacinth (Eichhornia crassipes) involving naturally isolated and recombinant enzymes and its recovery. Environ Prog. Sustain. 33:1396-1404. 

Ghosh, A., Verma, A.K., Luis, A.S., Bras, J.L.A., Fontes, C.M., Goyal, A., 2014. Mannan specific family 35 carbohydrate-binding module (CtCBM35) of Clostridium thermocellum: structure analysis and ligand binding. Biologia 69:1271-1282. 

Hamberg, Y., Ruimy-Israeli, V., Dassa, B., Barak, Y., Lamed, R., Cameron, K., Fontes, C.M.G.A., Bayer, E.A., Fried, D.B., 2014. Elaborate cellulosome architecture of Acetivibrio cellulolyticus revealed by selective screening of cohesin-dockerin interactions. PeerJ 2:e636

Nunes, C.I.P., Bras, J.L.A., Najmudin, S., Moura, J.J.G., Moura, I., Carepo, M.S.P., 2014. ArsC3 from Desulfovibrio alaskensis G20, a cation and sulfate-independent highly efficient arsenate reductase. J. Biol. Inorg. Chem. 19:1277-1285.

Ribeiro, T., Lordelo, M.M., Costa, P., Alves, S.P., Benevides, W.S., Bessa, R.J.B., Lemos, J.P.C., Pinto, R.M.A., Ferreira, L.M.A., Fontes, C.M.G.A., Prates, J.A.M., 2014. Effect of reduced dietary protein and supplementation with a docosahexaenoic acid product on broiler performance and meat quality. Br. Poultry Sci. 55:752-765. 

Venditto, I., Centeno, M.S.J., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S., 2014. Expression, purification and crystallization of a novel carbohydrate-binding module from the Ruminococcus flavefaciens cellulosome. Acta Crystallogr. F 70:1653-1656. 

Venditto, I., Santos, H., Sandy, J., Sanchez-Weatherby, J., Ferreira, L.M.A., Sakka, K., Fontes, C.M.G.A., Najmudin, S., 2014. Crystallization and preliminary X-ray diffraction analysis of a trimodular endo-beta-1,4-glucanase (Cel5B) from Bacillus halodurans. Acta Crystallogr. F 70:1628-1630. 

Bule, P., Ruimy-Israeli, V., Cardoso, V., Bayer, E.A., Fontes, C.M.G.A., Najmudin, S. 2014. Overexpression, crystallization and preliminary X-ray characterization of Ruminococcus flavefaciens scaffoldin C cohesin in complex with a dockerin from an uncharacterized CBM-containing protein. Acta Crystallogr. F Struct. Biol. Commun. 70:1061-1064.

Bule, P., Correia, A., Cameron, K., Alves, V.D., Cardoso, V., Fontes, C.M.G.A., Najmudin, S. 2014. Overexpression, purification, crystallization and preliminary X-ray characterization of the fourth scaffoldin A cohesin from Acetivibrio cellulolyticus in complex with a dockerin from a family 5 glycoside hydrolase. Acta Crystallogr. F Struct. Biol. Commun. 70:1065-1067.

Cameron, K., Alves, V.D., Bule, P., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S. 2014. Purification, crystallization and preliminary X-ray characterization of the third ScaB cohesin in complex with an ScaA X-dockerin from Acetivibrio cellulolyticus. Acta Crystallogr. F Struct. Biol. Commun. 70:656–658.

Costa, M., Fernandes, V.O., Ribeiro, T., Serrano, L., Cardoso, V., Santos, H., Lordelo, M., Ferreira, L.M.A., Fontes, C.M.G.A. 2014. Construction of GH16 β-Glucanase Mini-cellulosomes to improve the nutritive value of barley-based diets for broilers. J. Agr. Food Chem. 62:7496-7506.

Costa, A.S.H., Bessa, R.J.B., Pires, V.M.R., Rolo, E.A., Pinto, R.M.A., Fontes, C., Prates, J.A.M. 2014. Is hepatic lipid metabolism of beef cattle influenced by breed and dietary silage level? BMC Vet. Res. 10.

Goyal, A., Ahmed, S., Fontes, C., Najmudin, S. 2014. Crystallization and preliminary X-ray crystallographic analysis of a novel alpha-L-arabinofuranosidase (CtGH43) from Clostridium thermocellum ATCC 27405. Acta Crystallogr. F Struct. Biol. Commun. 70:616-618.

Lopes, P.A., Costa, A.S., Costa, P., Pires, V.M., Madeira, M.S., Achega, F., Pinto, R.M., Prates, J.A.M. 2014. Contrasting cellularity on fat deposition in the subcutaneous adipose tissue and longissimus lumborum muscle from lean and fat pigs under dietary protein reduction. Animal 8:629-637.

Madeira, M.S., Pires, V.M.R., Alfaia, C.M., Luxton, R., Doran, O., Bessa, R.J.B., Prates, J.A.M. 2014. Combined effects of dietary arginine, leucine and protein levels on fatty acid composition and gene expression in the muscle and subcutaneous adipose tissue of crossbred pigs. Br. J. Nut. 111:1521-1535.

Venditto, I., Santos, H., Ferreira, L.M.A., Sakka, K., Fontes, C., Najmudin, S., 2014. Overproduction, purification, crystallization and preliminary X-ray characterization of the family 46 carbohydrate-binding module (CBM46) of endo-beta-1,4-glucanase B (CelB) from Bacillus halodurans. Acta Crystallogr. F Struct. Biol. Commun. 70:754-757.

 

2013


Ahmed, S., Luis, A.S., Bras, J.L.A. Ghosh, A., Gautam, S., Gupta, M.N., Fontes, C.M.G.A., Goyal, A. 2013. A Novel α-L-Arabinofuranosidase of family 43 glycoside hydrolase (Ct43Araf) from Clostridium thermocellum. PLoS ONE, 8:73575.

Ahmed, S., Luis, A.S., Bras, J.L.A., Fontes, C., Goyal, A. 2013. Functional and structural characterization of family 6 carbohydrate-binding module (CtCBM6A) of Clostridium thermocellum alpha-L-arabinofuranosidase. Biochemistry-Moscow 78:1272-1279.

Ahmed, S., Luis, A.S., Bras, J.L.A., Fontes, C.M.G.A., Goyal, A. 2013. The family 6 carbohydrate-binding module (CtCBM6B) of Clostridium thermocellum alpha-L-arabinofuranosidase binds xylans and thermally stabilized by Ca2+ ions. Biocatal. Biotransfor. 31:217-225.

Costa, A.S.H., Costa, P., Bessa, R.J.B., Lemos, J.P.C., Simões, J.A., Santos-Silva, J., Fontes, C.M.G.A., Prates, J.A.M. 2013. Carcass fat partitioning and meat quality of Alentejana and Barrosã young bulls fed high or low maize silage diets. Meat Sci. 93: 405-412.

Costa, A.S.H., Pires, V.M.R., Fontes, C.M.G.A., Prates, J.A.M. 2013. Expression of genes controlling fat deposition in two genetically diverse beef cattle breeds fed high or low silage diets. BMC Vet. Res. 9:118.

Costa, A.S.H., Silva MP, Alfaia CPM, Pires VMR, Fontes, C.M.G.A., Bessa, R.J.B., Prates, J.A.M. 2013. Genetic background and diet impact beef fatty acid composition and stearoyl-CoA desaturase mRNA expression. Lipids 48:369-381.

Currie, M.A, Cameron, K., Dias, F.M.V., Spencer, H.L., Bayer, E.D., Fontes, C.M.G.A., Smith, S.P., Jia, Z. 2013. Small angle x-ray scattering analysis of clostridium thermocellum cellulosome N-terminal complexes reveals a highly dynamic structure. J. Biol. Chem. 288:7978-7985.

Das, S.P., Ravindran, R., Ghosh, A., Deka, D., Das, D., Jawed, M., Fontes, C.M.G.A., Goyal, A. 2013. Efficient pretreatment for bioethanol production from water hyacinth (eichhornia crassipes) involving naturally isolated and recombinant enzymes and its recovery. Environ. Prog. Sustain. 167:1475-1488.

Ghosh, A., Luis, A.S., Bras, J.L.A., Fontes, C., Goyal, A. 2013. Thermostable recombinant beta-(1,4)-mannanase from C-thermocellum: Biochemical characterization and manno-oligosaccharides production. J. Agr. Food Chem. 61: 12333-12344.

Ghosh, A., Luis, A.S., Bras, J.L.A., Pathaw, N., Chrungoo, N.K., Fontes, C., Goyal, A. 2013. Deciphering ligand specificity of a Clostridium thermocellum family 35 carbohydrate binding module (CtCBM35) for gluco- and galacto-substituted mannans and its Calcium induced stability. PLoS ONE 8:80415.

Luís, A.S., Venditto, I., Temple, M.J., Rogowski, A., Baslé, A., Xue, J., Knox, J.P., Prates, J.A.M., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S., Gilbert, H.J. 2013. Understanding how noncatalytic carbohydrate binding modules can display specificity for xyloglucan. J. Biol. Chem. 288: 4799-4809.

Madeira, M.S., Pires, V.M., Alfaia, C.M., Costa, A.S., Luxton, R., Doran, O., Bessa, R.J.B., Prates, J.A.M. 2013. Differential effects of reduced protein diets on fatty acid composition and gene expression in muscle and subcutaneous adipose tissue of Alentejana purebred and Large White × Landrace × Pietrain crossbred pigs. Br. J. Nutr. 110:216-229.

Mendes, A.R., Ribeiro, T., Correia, B.A., Bule, P., Maçãs, B., Falcão, L., Freire, J.P.B., Ferreira, L.M.A., Fontes, C.M.G.A., Lordelo, M.M. 2013. Low doses of exogenous xylanase improve the nutritive value of triticale-based diets for broilers. J. Appl. Poultry Res. 22:92-99.

Pimenta, J., Sardinha, J., Marques, C.C., Domingos, A., Baptista, M.C., Barbas, J.P., Martins, I.C., Mesquita, P., Pessa, P., Soares, R., Viegas, A., Cabrita, E., Horta, A.E.M., Fontes, C.M.G.A., Prates, J.A.M., Pereira, R.M. 2013. Inhibition of ovine in vitro fertilization by anti-Prt antibody: Hypothetical model for Prt/ZP interaction. Reprod. Biol. Endocrinol. 11:25

Pimenta, J., Viegas, A., Sardinha, J., Martins, I.C., Cabrita, E.J., Fontes, C.M.G.A., Prates, J.A.M., Romão, M.L.N. 2013. NMR solution structure and SRP54M predicted interaction of the N-terminal sequence (1-30) of the ovine Doppel protein. Peptides 49:32-40.

Ribeiro, T., Lordelo, M.M., Alves, S.P., Bessa, R.J.B., Costa, P., Lemos, J.P.C., Ferreira, L.M.A., Fontes, C.M.G.A., Prates, J.A.M. 2013. Direct supplementation of diet is the most efficient alternative to enrich broilers meat with n-3 long-chain polyunsaturated fatty acids. Br. Poultry Sci. 56:753-765.

Santos, C.I., Ribeiro, T., Ponte, P.I.P., Fernandes, V.O., Falcão, L., Freire, J.P., Prates, J.A.M., Ferreira, L.M.A., Fontes, C.M.G.A., Lordelo, M.M. 2013. The effects of restricting enzyme supplementation in rye-based diets for broilers. Anim. Feed Sci. Tech. 186:214- 217.

Venditto, I., Baslé, A., Luís, A.S., Temple, M.J., Ferreira, L.M.A., Fontes, C.M.G.A., Gilbert, H.J., Najmudin, S. 2013. Overproduction, purification, crystallization and preliminary X-ray characterization of the C-terminal family 65 carbohydrate-binding module (CBM65B) of endoglucanase Cel5A from Eubacterium cellulosolvens. Acta Crystallogr. F Struct. Biol. Commun. 69:191-194.

Verma, A.K., Goyal, A., Freire, F., Bule, P., Venditto, I., Brás, J.L.A., Santos, H., Cardoso, V., Bonifácio, C., Thompson, A., Romão, M.J., Prates, J.A.M., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S. 2013. Overexpression, crystallization and preliminary X-ray crystallographic analysis of glucuronoxylan xylanohydrolase (Xyn30A) from Clostridium thermocellum. Acta Crystallogr. F Struct. Biol. Commun. 69:1440–1442.

Viegas, A., Sardinha, J., Freire, F., Duarte, D.F., Carvalho, A.L., Fontes, C.M.G.A., Romão, M.J., Macedo, A.L., Cabrita, E.J. 2013. Solution structure, dynamics and binding studies of a family 11 carbohydrate-binding module from clostridium thermocellum (Ct CBM11). Biochem. J. 451:289-300.

 

2012


Palma, A.S., Liu, Y., Zhang, Y.B., Zhang, H.T., Luis, A.S., Carvalho, A.L., Gilbert, H.J., Boraston, A., Fontes, C., Chai, W.G., Ten, F.Z. 2012. Designer-oligosaccharide microarrays to decipher ligands in mammalian and prokaryotic glucan-recognition systems. Glycobiology 22, 1612-1613.

Brás, J.L.A., Alves, V.D., Carvalho, A.L., Najmudin, S., Prates, J.A.M., Ferreira, L.M.A., Bolam, D.N., Fontes, C.M.G.A. 2012. Novel Clostridium thermocellum Type I cohesin-dockerin complexes reveal a single binding mode. J. Biol. Chem. 287:44394-44405.

Brás, J.L.A., Carvalho, A.L., Viegas, A., Nadjmudim, S., Alves, V.D., Prates, J.A.M., Ferreira, L.M.A., Romão, M.J., Gilbert, H.J., Fontes, C.M.G.A. 2012. Escherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexes. Method. Enzymol. 510:395-415.

Cameron, K., Alves, V.D., Bule, P., Ferreira, L.M.A., Fontes, C.M.G.A., Najmudin, S. 2012. Purification, crystallization and preliminary X-ray characterization of the Acetivibrio cellulolyticus type I cohesin ScaC in complex with the ScaB dockerin. Acta Crystallogr. F Struct. Biol. Commun. 68:1030-1033.

Costa, A.S.H., Lopes, P.A., Estevão, M., Martins, S.V., Alves, S.P., Pinto, R.M.A., Pissarra, H., Correia, J.J., Pinho, M., Fontes, C.M.G.A., Prates, J.A.M. 2012. Contrasting cellularity and fatty acid composition in fat depots from alentejana and barrosã bovine breeds fed high and low forage diets. Int. J. Biol. Sci. 8:214-227.

Das, S.P., Ravindran, R., Ahmed, S., Das, D., Goyal, D., Fontes, C.M.G.A., Goyal, A. 2012. Bioethanol production involving recombinant C. thermocellum hydrolytic hemicellulase and fermentative microbes. Appl. Biochem. Biotech. 167:1475-1488.

Figueiredo, A.A., Correia, B.A., Ribeiro, T., Ponte, P.I.P., Falcão, L., Freire, J.P., Prates, J.A.M., Ferreira, L.M.A., Fontes, C.M.G.A., Lordelo, M.M. 2012. The effects of restricting enzyme supplementation in wheat-based diets to broilers. Anim. Feed Sci.Tech. 172:194-200.

Fontes, C. M. G. A., Correia, I. S., Romao, M. J., Sa-Nogueira, I., Prates, J. A. M., Ferreira, L. M. A. 2012. Editorial of the 9th Carbohydrate bioengineering meeting. Biocatal. Biotransfor. 30: 273-273.

Mizutani, K., Fernandes, V.O., Karita, S., Luís, A.S., Sakka, M., Kimura, T., Jackson, A., Zhang, X., Fontes, C.M.G.A., Gilbert, H.J., Sakka, K. 2012. Influence of a mannan binding family 32 carbohydrate binding module on the activity of the appended mannanase. Appl. Environ. Microb. 78:4781-4787.

Pimenta, J., Dias, F., Marques, C., Tista, M., Vasques, M., Horta, A., Barbas, J., Soares, R., Mesquita, P., Cabrita, E., Fontes, C.M.G.A., Prates, J.A.M., Pereira, R. 2012. The prion-like protein Doppel enhances ovine spermatozoa fertilizing ability. Reprod. Domest. Anim. 47:196-202.

Pimenta, J., Domingos, A., Santos, P., Marques, C., Cantante, C., Santos, A., Barbas, J., Tista, M., Horta, A., Viegas, A., Mesquita, P., Gonçalves, J., Fontes, C.M.G.A., Prates, J.A.M., Pereira, R. 2012. Is prnt a pseudogene? Identification of ram Prt in testis and ejaculated spermatozoa. PLoS ONE 7:42957.

Pinheiro, B.A., Bras, J.L.A., Najmudin, S., Carvalho, A.L., Ferreira, L.M.A., Prates, J.A.M., Fontes, C. 2012. Flexibility and specificity of the cohesin-dockerin interaction: implications for cellulosome assembly and functionality. Biocatal. Biotransfor. 30:309-315.

Ribeiro T., Lordelo, M.M.S., Prates, J.A.M., Falcão, L., Freire, J.P.B., Ferreira, L.M.A., Fontes, C.M.G.A. 2012. The thermostable β-1,3-1,4-glucanase from Clostridium thermocellum improves the nutritive value of highly viscous barley-based diets for broilers. Br. Poultry Sci. 2:224-234.

 

2011


Brás, J.L.A., Cartmell, A., Carvalho, A.L., Verzé, G., Bayer, E.A., Vazana, Y., Correia, M.A., Prates, J.A.M., Ratnaparkhe, S., Boraston, A.B., Romão, M.J., Fontes, C.M.G.A., Gilbert, H.J. 2011. Structural insights into a unique cellulase fold and mechanism of cellulose hydrolysis. P. Natl. Acad. Sci. USA 108:5237-5242.

Brás, J.L.A., Correia, M.A.S., Romão, M.J., Prates, J.A.M., Fontes, C.M.G.A., Najmudin, S. 2011. Purification, crystallization and preliminary X-ray characterization of the of penta-modular arabinoxylanase CtXyl5A from Clostridium thermocellum. Acta Crystallogr. F Struct. Biol. Commun. 67:833-836.

Correia, M.A.S., Mazumder, K., Bras, J.L.A., Firbank, S.J., Zhu, Y.P., Lewis, R.J., York, W.S., Fontes, C., Gilbert, H.J. 2011. Structure and function of an arabinoxylan-specific xylanase. J. Biol. Chem. 286:22510-22520.

Garcia-Alvarez, B., Melero, R., Dias, F.M.V., Prates, J.A.M., Fontes, C., Smith, S.P., Romao, M.J., Carvalho, A.L., Llorca, O. 2011. Molecular architecture and structural transitions of a Clostridium thermocellum mini-cellulosome. J. Mol. Biol. 407:571-580.

Luis, A.S., Alves, V.D., Romao, M.J., Prates, J.A.M., Fontes, C., Najmudin, S. 2011. Overproduction, purification, crystallization and preliminary X-ray characterization of a novel carbohydrate-binding module of endoglucanase Cel5A from Eubacterium cellulosolvens. Acta Crystallogr. F Struct. Biol. Commun. 67:491-493.

Montanier, C., Correia, M., Flint, J., Zhu, Y., Mckee, L., Prates, J.A.M., Polizzi, S., Coutinho, P., Lewis, R., Henrissat, B., Fontes, C.M.G.A., Gilbert, H.J. 2011. A novel, non-catalytic carbohydrate-binding module displays specificity for galactose-containing polysaccharides through calcium-mediated oligomerization. J. Biol. Chem. 286:22499-22509.

Ribeiro, T., Lordelo, M.M.S., Ponte, P.I.P., Macas, B., Prates, J.A.M., Fontes, M.A., Falcao, L., Freire, J.P.B., Ferreira, L.M.A., Fontes, C.M.G.A. 2011. Levels of endogenous β-glucanase activity in barley affect the efficacy of exogenous enzymes used to supplement barley-based diets for poultry. Poultry Sci. 90:1245-1256.

Sossidou, E.N., Dal Bosco, A., Elson, H.A., Fontes, C. 2011. Pasture-based systems for poultry production: implications and perspectives. World Poultry Sci. J. 67:47-58.

 

2010


Alfaia, C.M.M., Alves, S.P., Lopes, A.F., Fernandes, M.J.E., Costa, A.S.H., Fontes, C., Castro, M.L.F., Bessa, R.J.B., Prates, J.A.M. 2010. Effect of cooking methods on fatty acids, conjugated isomers of linoleic acid and nutritional quality of beef intramuscular fat. Meat Sci. 84:769-777.

Correia, M.A.S., Abbott, D.W., Gloster, T.M., Fernandes, V.O., Prates, J.A.M., Montanier, C., Dumon, C., Williamson, M.P., Tunnicliffe, R.B., Liu, Z.Y., Flint, J.E., Davies, G.J., Henrissat, B., Coutinho, P.M., Fontes, C., Gilbert, H.J. 2010. Signature active site architectures illuminate the molecular basis for ligand specificity in family 35 carbohydrate binding module. Biochemistry 49:6193-6205.

Fontes, C., Gilbert, H.J. 2010. Cellulosomes: Highly efficient nanomachines designed to deconstruct plant cell wall complex carbohydrates. In: Kornberg, R.D., Raetz, C.R.H., Rothman, J.E., Thorner, J.W. (Eds.), Annu. Rev. Biochem. 79:655-681.

Mesquita, P., Batista, M., Marques, M.R., Santos, I., Pimenta, J., Pereira, M., Carolino, I., Santos-Silva, F., Sousa, M., Gama, L.T., Fontes, C.M.G.A., Horta, A.E.M., Prates, J.A.M., Pereira, R.M. 2010. Prion-like Doppel gene polymorphisms and scrapie susceptibility in portuguese sheep breeds. Anim. Genet. 41:311-314.

Najmudin, S.H., Pinheiro, B.A., Prates, J.A.M., Gilbert, H.J., Romão, M.J., Fontes, C.M.G.A. 2010. Putting an N-terminal end to the Clostridium thermocellum xylanase Xyn10B story: Crystal structure of the CBM22-1-GH10 modules complexed with xylohexaose. J. Struct. Biol. 172:353-362.

Ribeiro, T., Silva, T.S., Alves, V.O.M., Dias, F.V.M., Luís, A.S., Prates, J.A.M., Ferreira, L.A.M., Romão, M.J., Fontes, C.M.G.A. 2010. Family 42 carbohydrate-binding modules display multiple arabinoxylan-binding interfaces presenting different ligand affinities. BBA - Proteins Proteom. 1804:2054-2062.

 

2009


Ahmed, S., Bharali, S., Purama, R.K., Majumder, A., Fontes, C., Goyal, A. 2009. Structural and biochemical properties of lichenase from Clostridium thermocellum. Indian J. Microbiol. 49:72-76.

Alfaia, C.P.M., Alves, S.P., Martins, S.I.V., Fontes, C.M.G.A., Lemos, J.P.C., Bessa, R.J.B., Prates, J.A.M. 2009. Effect of the feeding system on intramuscular fatty acids and conjugated linoleic acid isomers of beef cattle, with emphasis on their nutritional value and discriminatory ability. Food Chem. 114:939-946.

Correia, M.A.S., Pires, V.M.R., Gilbert, H.J., Bolam, D.N., Fernandes, V.O., Alves, V.D., Prates, J.A.M., Ferreira, L.M.A., Fontes, C.M.G.A. 2009. Family 6 Carbohydrate-Binding Modules display multiple b-1,3-linked glucan specific binding interfaces. FEMS Microbiol. Lett. 300:48-57.

Montanier, C., Money, V.A., Pires, V.M.R., Flint, J.E., Pinheiro, B.A., Goyal, A., Prates, J.A.M., Izumi, A., Stalbrand, H., Morland, C., Cartmell, A., Kolenova, K., Topakas, E., Dodson, E.J., Bolam, D.N., Davies, G.J., Fontes, C., Gilbert, H.J. 2009. The active site of a carbohydrate esterase displays divergent catalytic and noncatalytic binding functions. PLoS Biol. 7:687-697.

Montanier, C., Bueren, A.L.V., Dumon, C., Flint, J.E., Correia, M.A., Prates, J.A., Firbank, S.J., Lewis, R.J., Grondin, G.G., Ghinet, M.G., Gloster, T.M., Hervef, C., Knox, J.P., Talbot, B.G., Turkenburg, J.P., Kerovuo, J., Brzezinski, R., Fontes, C.M.G.A., Davies, G.J., Boraston, A.B., Gilbert, H.J. 2009. Evidence that family 35 carbohydrate-binding modules display conserved specificity but divergent function. P. Natl. Acad. Sci. USA. 106:3065–3070.

Pereira, R.M., Mesquita, P., Batista, M., Baptista, M.C., Barbas, J.P., Pimenta, J., Santos, L.C., Marques, M.R., Vasques, M.I., Silva-Pereira, M., Santos-Silva, F., Oliveira-Sousa, M.C., Fontes, C.M.G., Horta, A.E.M., Prates, J.A.M., Marques, C.C. 2009. Doppel gene polymorphisms in Portuguese sheep breed: Insights on ram fertility. Anim. Reprod. Sci. 114:157-166.

Pinheiro, B.A., Gilbert, H.J., Sakka, K., Fernandes, V.O., Prates, J.A.M., Alves, V.D., Bolam, D.N., Ferreira, L.M.A., Fontes, C. 2009. Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum. Biochem. J. 424:375-384.

 

Book Chapters


 

Alves, V.D., Fontes, C.M.G.A., Bule, P., 2021. Cellulosomes: Highly Efficient Cellulolytic Complexes, in: Harris, J.R., Marles-Wright, J. (Eds.), Macromolecular Protein Complexes III: Structure and Function, Subcellular Biochemistry. Springer International Publishing, Cham, pp. 323–354.

Bule, P., Najmudin, S., Brás, J., Pires, V., Fernandes, V., Sequeira, A., Cameron, K., Leitão, A., Prates, J.A.M., Ferreira, L.M.A., Alves, V.D., Fontes, C.M.G.A., 2020. The Fine Structure of the Cellulosome Defines the Intricacies of Carbohydrate Deconstruction in the Mammalian Gut, in: Freitas Duarte, A., Lopes da Costa, L. (Eds.), Advances in Animal Health, Medicine and Production. Springer International Publishing, Cham, pp. 87–107.

Cardoso, V., Ribeiro, T., Fernandes, V., Guerreiro, C., Centeno, M., Pires, V., Ponte, P., Goyal, A., Najmudin, S., Alves, V.D., Prates, J.A.M., Ferreira, L.M.A., Fontes, C.M.G.A., 2020. Exogenous Enzymes Improve the Nutritive Value of Cereal-Based Diets for Monogastric Animals Through Different Mechanisms, in: Freitas Duarte, A., Lopes da Costa, L. (Eds.), Advances in Animal Health, Medicine and Production. Springer International Publishing, Cham, pp. 108–127.

Brás, J.L.A., Carvalho, A.L., Viegas, A., Nadjmudim, S., Alves, V.D., Prates, J.A.M.,  Ferreira, L.M.A., Romão, M.J., Gilbert, H.J., Fontes, C.M.G.A. 2012. Escherichia coli expression, purification, crystallization and structure determination of bacterial cohesin-dockerin complexes. In: Enzymol. 510:395-415. Ed. H. Gilbert. Academic Press, Dan Diego, USA. ISBN 978-012415-931-0.